Journal: Cell Reports Medicine

Article Title: A 211 At-labelled mGluR1 inhibitor induces cancer senescence to elicit long-lasting anti-tumor efficacy

doi: 10.1016/j.xcrm.2023.100960

Figure Lengend Snippet:

Article Snippet: Commercially available senescence-associated β-galactosidase (SA-β-gal) staining kits (SG02, Dojindo) were used to detect senescence in cells and tissues as instructed by the manufacturer.

Techniques: Recombinant, Plasmid Preparation, Staining, Luminex, RNA Sequencing, Software, Cell Counting

Journal: Biomedicines

Article Title: Isoproterenol Induces Cardiac Injury and Senescence in Sprague–Dawley Rats: A Cost-Effective Pharmacological Model

doi: 10.3390/biomedicines14071445

Figure Lengend Snippet: ISO induces senescence in the apex of the young adult rat. 10–12-week-old rats were administered 100 mg/kg ISO or saline for five days, and then apexes were collected on days 10 and 28. ( A ) Representative SA-β-gal staining images (blue; black arrows). ( B ) Quantification of SA-β-gal-positive area. ( C ) Representative p16 staining images (green; yellow arrows). ( D ) Quantification of p16-positive cells. ( E ) Representative p21 staining images (green; yellow arrows). ( F ) Quantification of p21-positive cells. Scale bars = 100 µm. Data are expressed as the mean ± S.D. Statistical analysis was performed using two-way ANOVA followed by Šídák’s multiple comparisons test. n = 5 per group.

Article Snippet: Frozen sections were used for SA-β-gal (HY-K1089, MedChemExpress, Monmouth Junction, NJ, USA) and p16 staining.

Techniques: Saline, Staining